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USA | MD | PRINCE GEORGES COUNTY | BELTSVILLE | 20704 :AGRICULTURE, DEPARTMENT OF- AGRICULTURAL RESEARCH SERVICE- USDA ARS AFM APD

Genomics

RNA Sequencing Service -
Documents

USA | DC | DISTRICT OF COLUMBIA COUNTY | WASHINGTON:ENVIRONMENTAL PROTECTION AGENCY- ENVIRONMENTAL PROTECTION AGENCY- CINCINNATI ACQUISITION DIV ( CAD)

Genomics

A-- High Throughput Transcriptomics ( HTTr) - AH91 - High Throughput Transcriptomics ( HTTr)
Documents

USA | MD | PRINCE GEORGES COUNTY | BELTSVILLE | 20704 :AGRICULTURE, DEPARTMENT OF- AGRICULTURAL RESEARCH SERVICE- USDA ARS AFM APD

Genomics

High- Throughput Sequencing Service - Amend **** Posted. This is a combined synopsis/solicitation for commercial item prepared in accordance with the format in FAR subpart 12. 6 and FAR Part 13, as supplemented with additional information included in this notice. This announcem
Documents

USA | CO | DENVER COUNTY | DENVER | 80264 :INTERIOR, DEPARTMENT OF THE- US GEOLOGICAL SURVEY- OFC OF ACQUISITION GRANTS- DENVER

Genomics

B-- DNA SEQUENCING SERVICES - DNA Sequencing Services for high throughput, short read sequencing services to generate whole genome data for native salmonids in the Great Lakes. This service includes quality control analysis, sequencing, and delivery of demultiplexed sequence dat

b529 special studies analysis scientific data scientific data studies dna sequencing services for high throughput, short read sequencing services to generate whole genome data for native salmonids in the great lakes. this service includes quality control analysis, sequencing, and delivery of demultiplexed sequence data from genomic dna libraries prepared by the usgs great lake science center.
Documents

USA | MD | PRINCE GEORGES COUNTY | BELTSVILLE | 20704 :AGRICULTURE, DEPARTMENT OF- AGRICULTURAL RESEARCH SERVICE- USDA ARS AFM APD

Genomics

RNA- Seq of 140 Samples - RFQ - i. This is a combined synopsis/solicitation for commercial items prepared in accordance with the procedures contained in FAR Part 12. 6, Acquisition of Commercial Items, and FAR Part 13, Simplified Acquisition Procedures, as supplemented with addit

RNAseq of 140 samples from the pig intestines 140 rna samples will be provided by arsabbl scientists to the contractor, who will in turn perform rna quality assessment, polya tail enrichment library construction, library qc, pooling, and normalization, and dna sequencing. 1. assess rna quality rna quality will be assessed using a method which determines both the quality and quantity of rna, including the differentiation of genomic dna and rna using electrophoresis. results of the rna quality testing will be compiled in a document for delivery to researchers. 2. prepare sequencing libraries high quality rna will be used to prepare sequencing libraries. libraries will be constructed via enrichment of polya tails, and the construction will be performed such that the directionality of the rna molecules is accounted for. qc of libraries will be performed. 3. pool and normalize libraries high quality libraries will be pooled in equal volumes and normalized. 4. sequence libraries normalized libraries will be sequenc
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